This study investigated into the relationship between students’ performance in item independent and item dependent tests in senior secondary school biology in Enugu North Local Government of Enugu State. A sample of 180 SS II students was drawn from four SS in Enugu North Local Government Area of Enugu State using simple random sampling technique. Two research questions were asked and two research hypotheses were formulated and tested at 0.05 level of significance. The instruments for data collection were biology item independent test (BIIT) and biology item dependent test (BIDT) which were ten items each. The reliability coefficients of (BIIT) and (BIDT) were computed as 0.78 and 0.81 respectively using Kuder-Richardson K-R20 formula. The result of the study shows that there is a high positive relationship between students’ (BIIT) and (BIDT) in biology. It was also found that there was an inverse relationship between male and female students in their performance in (BIIT) and (BIDT) biology. It is recommended that teachers should be encouraged to construct test item on (BIIT) and (BIDT) for biology students.
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